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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps add to tumorigenesis. This protein was located to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a important part in advertising mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been explained. Two further splice variants were claimed but thorough nucleotide sequence info is not really nevertheless accessible.
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Blocking peptides are peptides that bind particularly to your concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope identified by the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to make certain the very best success are...
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This guide outlines further more doable optimization of cellular and immunoassay parameters to ensure the very best final results are...
Antibodies certain to the blocking peptide no longer bind to your epitope on the concentrate on protein. By evaluating the staining within the blocked antibody compared to the antibody by itself, one can see which staining is specific.
Focus on Info Cyclin E belongs to your very conserved cyclin family, whose customers are characterised by a spectacular periodicity in protein abundance through the cell cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins show distinct expression and degradation designs which add to the temporal coordination of every mitotic party. Cyclin E types a fancy with and capabilities as being a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S transition. This protein accumulates on the G1-S period boundary and is particularly degraded as cells progress by S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both equally antibodies...
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